• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
唐嘉诚,包建强,陈彦婕,等. 大鲵黏液糖蛋白的提取工艺优化及纯化表征[J]. 食品工业科技,2022,43(21):252−261. doi: 10.13386/j.issn1002-0306.2022030284.
引用本文: 唐嘉诚,包建强,陈彦婕,等. 大鲵黏液糖蛋白的提取工艺优化及纯化表征[J]. 食品工业科技,2022,43(21):252−261. doi: 10.13386/j.issn1002-0306.2022030284.
TANG Jiacheng, BAO Jianqiang, CHEN Yanjie, et al. Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander[J]. Science and Technology of Food Industry, 2022, 43(21): 252−261. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030284.
Citation: TANG Jiacheng, BAO Jianqiang, CHEN Yanjie, et al. Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander[J]. Science and Technology of Food Industry, 2022, 43(21): 252−261. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030284.

大鲵黏液糖蛋白的提取工艺优化及纯化表征

Extraction Process Optimization,Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander

  • 摘要: 本文以丽水人工养殖大鲵的黏液为原料,优化大鲵黏液粗糖蛋白提取工艺并进行纯化表征。基于单因素实验,以料液比、温度、提取液浓度以及提取时间为考察因素,粗糖蛋白得率为指标,采用响应面法优化提取工艺,得到最佳提取条件为:料液比1:30.5 g/mL、提取温度51 ℃、提取液浓度0.32 mol/L、提取时间5.2 h,大鲵黏液粗糖蛋白得率最高为5.22%±0.71%。纯化得到大鲵黏液糖蛋白(MGP),经表征分析,测定其达到电泳纯,分子量约为29 kDa;MGP中必需氨基酸及药用氨基酸含量分别为37.21 g/100 g和33.39 g/100 g;MGP具有典型的糖蛋白结构的紫外全波长吸收图像;含有O型糖肽键,吡喃糖环结构和β-糖苷键,β-折叠结构占蛋白质二级结构的87%,为后续深入探究大鲵黏液糖蛋白生物活性提供一定基础。

     

    Abstract: The extraction process of crude glycoprotein from Chinese giant salamander (MGP) mucus cultured in Lishui was optimized. The crude glycoprotein was further purified, of which characterization was analysed. Based on single factor experiment, the extraction process was optimized by response surface methodology with solid-liquid ratio, temperature, extraction concentration, extraction time as factors, and glycoprotein yield as index. The results showed that the optimal extraction conditions were solid-liquid ratio of 1:30.5 g/mL, extraction temperature of 51 ℃, extraction concentration of 0.32 mol/L, extraction time of 5.2 h, the highest yield of MGP was 5.22%±0.71%, which was basically consistent with the expected results. The electrophoresis pure of purified glycoprotein was determined. The lyophilized MGP was white, loose powder, of which molecular weight was about 29 kDa. The contents of essential amino acids and medicinal amino acids in MGP were 37.21 g/100 g and 33.39 g/100 g, respectively. MGP had a typical glycoprotein structure of UV full wavelength absorption image, containing o-glycopeptide bond, pyranose ring structure and β-glycosidic bond. The secondary structure of MGP was mainly composed of 87% β-folded, which provided a theoretical basis for further research on the biological activity of glycoprotein from the mucus of Chinese giant salamander.

     

/

返回文章
返回