• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
陈永,宋真萍,吴晖,等. 红蓝草多糖提取工艺优化及其抗氧化活性分析[J]. 食品工业科技,2023,44(5):166−173. doi: 10.13386/j.issn1002-0306.2022050191.
引用本文: 陈永,宋真萍,吴晖,等. 红蓝草多糖提取工艺优化及其抗氧化活性分析[J]. 食品工业科技,2023,44(5):166−173. doi: 10.13386/j.issn1002-0306.2022050191.
CHEN Yong, SONG Zhenping, WU Hui, et al. Optimization of the Extraction Process of Peristrophe roxburghiana Polysaccharide and Analysis of Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2023, 44(5): 166−173. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050191.
Citation: CHEN Yong, SONG Zhenping, WU Hui, et al. Optimization of the Extraction Process of Peristrophe roxburghiana Polysaccharide and Analysis of Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2023, 44(5): 166−173. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050191.

红蓝草多糖提取工艺优化及其抗氧化活性分析

Optimization of the Extraction Process of Peristrophe roxburghiana Polysaccharide and Analysis of Its Antioxidant Activity

  • 摘要: 为探究红蓝草多糖的最佳提取工艺及其体外抗氧化活性。试验以红蓝草为原料,探究料液比、浸提温度、浸提时间、浸提次数对红蓝草多糖得率的影响,并结合响应面法优化红蓝草多糖提取工艺,通过测定红蓝草多糖对DPPH·、·OH、ABTS+·等自由基的清除能力探究其抗氧化活性。结果表明:在料液比1:31 g/mL、浸提温度85 ℃、浸提时间118 min、提取3次的条件下,红蓝草多糖得率最高,可达11.05%。在一定范围内,红蓝草多糖清除自由基能力与多糖的浓度呈量效关系,红蓝草多糖溶液清除DPPH ·· OH和ABTS + ·等自由基的IC50值分别为0.18、0.73、0.64 mg/mL,说明红蓝草粗多糖具有一定的抗氧化活性。通过探究红蓝草多糖提取的最佳工艺及抗氧化活性,为今后红蓝草多糖的进一步开发与应用提供理论基础和参考。

     

    Abstract: In order to explore the optimum extraction process of polysaccharide and antioxidant activity in vitro in Peristrophe roxburghiana. In this study, Peristrophe roxburghiana was used as raw material to explore the effects of solid-liquid ratio, extraction temperature, time and frequency on the yield of polysaccharide. The extraction process of polysaccharide in Peristrophe roxburghiana was optimized by response surface methodology and the antioxidant activity of the polysaccharide was investigated by measuring its scavenging ability on DPPH·, ·OH, ABTS+· and other free radicals. The results showed that we could get the most quantity of polysaccharide extracted from Peristrophe roxburghiana, which reaches up to 11.05%, in these conditions: The solid-liquid ratio was 1:31 g/mL, the extraction temperature was 85 ℃, the extraction time was 118 min and the extraction frequency was 3 times. The scavenging ability of the polysaccharide against free radical was quantitatively related to the concentration of polysaccharide within a certain range. The IC50 values of DPPH·, ·OH and ABTS+· radicals scavenged by the polysaccharide solution were 0.18, 0.73 and 0.64 mg/mL, respectively, indicating that the polysaccharide extracted from Peristrophe roxburghiana was with certain antioxidant activity. By exploring the optimal process and antioxidant activity of the polysaccharide extracted from Peristrophe roxburghiana, we provide a theoretical basis and reference for the further development and application of the polysaccharide in the future.

     

/

返回文章
返回