• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
叶茂,沈晓玲,陈青舟,等. 胶体金免疫层析法同时检测果蔬中四种农药残留[J]. 食品工业科技,2023,44(6):300−308. doi: 10.13386/j.issn1002-0306.2022050323.
引用本文: 叶茂,沈晓玲,陈青舟,等. 胶体金免疫层析法同时检测果蔬中四种农药残留[J]. 食品工业科技,2023,44(6):300−308. doi: 10.13386/j.issn1002-0306.2022050323.
YE Mao, SHEN Xiaoling, CHEN Qingzhou, et al. Simultaneous Determination of Four Pesticide Residues in Fruits and Vegetables by Colloidal Gold Immunochromatography[J]. Science and Technology of Food Industry, 2023, 44(6): 300−308. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050323.
Citation: YE Mao, SHEN Xiaoling, CHEN Qingzhou, et al. Simultaneous Determination of Four Pesticide Residues in Fruits and Vegetables by Colloidal Gold Immunochromatography[J]. Science and Technology of Food Industry, 2023, 44(6): 300−308. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050323.

胶体金免疫层析法同时检测果蔬中四种农药残留

Simultaneous Determination of Four Pesticide Residues in Fruits and Vegetables by Colloidal Gold Immunochromatography

  • 摘要: 目的:研制一种胶体金免疫层析四线卡,可同时检测果蔬中毒死蜱、氟虫腈、克百威、多菌灵4种农药残留。方法:以硝酸纤维素膜为载体,农药竞争抗原为包被抗原,胶体金为标记材料,制备胶体金免疫层析四线卡,并对标记抗体浓度、检测线包被浓度、样品提取剂、提取时间等参数条件进行优化,评价四线卡的灵敏度、特异性、稳定性和准确性。结果:优化后四线卡制备工艺参数中毒死蜱、氟虫腈、克百威、多菌灵的最适标记抗体浓度为3.2、2.4、3.2、3.2 µg/mL,T1、T2、T3、T4抗原包被浓度分别为0.65、0.80、0.65、0.5 mg/mL。毒死蜱检出限为0.1 mg/kg、氟虫腈为0.02 mg/kg、克百威为0.02 mg/kg、多菌灵为0.05 mg/kg,除了克百威与丁硫克百威的交叉反应率为20%,毒死蜱、氟虫腈和多菌灵与结构类似物的交叉反应率均小于5%,在室温条件下可保存至少12个月。经验证,对60份果蔬样品检测结果与仪器法检测结果一致。结论:本研究制备的四线卡操作简便、准确度高、快速、稳定,可以用于果蔬中毒死蜱、氟虫腈、克百威、多菌灵残留的现场快速筛查和检测。

     

    Abstract: Objective: In this study, a colloidal gold immunochromatographic four lines card for the simultaneous determination of chlorpyrifos, fipronil, carbofuran and carbendazim in fruits and vegetables was prepared. Methods: The four lines card was prepared by using nitrocellulose membrane as carrier, pesticide competitive antigen as coating antigen and colloidal gold as labeling material. The parameter conditions, including concentrations of antibody for labeling, concentration of the coating antigen, sample extractant and extraction time were optimized. Under the optimal condition, the sensitivity, specificity and stability, as well as the accuracy of the four lines card were evaluated. Results: In the optimized of four lines card preparation process parameters, the concentration of colloidal gold labeled antibody of chlorpyrifos, fipronil, carbofuran and carbendazim were 3.2, 2.4, 3.2 and 3.2 µg/mL, and the concentration of antigen coating of T1, T2, T3 and T4 were 0.65, 0.80, 0.65 and 0.5 mg/mL, respectively. The limit of detection of chlorpyrifos was 0.1 mg/kg, fipronil was 0.02 mg/kg, carbofuran was 0.02 mg/kg, and carbendazim was 0.05 mg/kg. Except that the cross-reaction rates of carbofuran with carbosulfan was 20%, the cross-reaction rates of chlorpyrifos, fipronil and carbendazim with six structural analogues were less than 5%. It could be stored at room temperature for at least 12 months. Furthermore, 60 samples were analyzed and the detection results were consistent with those obtained in reference method. Conclusion: The four lines card prepared in this study was simple, accurate, fast and stable, which was suitable for the rapid screening and detection of chlorpyrifos, fipronil, carbofuran and carbendazim residues in fruits and vegetables.

     

/

返回文章
返回