Promoter P43 regulatory effect for subtilosin A synthesis by expressing key enzyme gene

SUN Hui-gang; ZHANG Chong; LV Feng-xia; BIE Xiao-mei; LU Zhao-xin

doi:10.13386/j.issn1002-0306.2013.23.002

SUN Hui-gang, ZHANG Chong, LV Feng-xia, BIE Xiao-mei, LU Zhao-xin. Promoter P43 regulatory effect for subtilosin A synthesis by expressing key enzyme gene[J]. Science and Technology of Food Industry, 2013, (23): 164-167. DOI: 10.13386/j.issn1002-0306.2013.23.002

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Promoter P43 regulatory effect for subtilosin A synthesis by expressing key enzyme gene

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Abstract

For improving Bacillus subtilis 168 bacteriocin subtilosin A production and applying to food preservation, medical science and so on, P 43 was linked to key enzyme gene sbo-albA, albB-albC of subtilosin A operon using SOE-PCR.High expressing vector was constructed.This recombinant vector was transformed into Bacillus subtilis 168 by high osmolarity electroporation method.HPLC assay showed subtilosin A production was improved.Inhibited method also showed the improving.This method will provide theory reference of improving subtilosin A production and applying in productive practice.

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Promoter P43 regulatory effect for subtilosin A synthesis by expressing key enzyme gene

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