Development of real-time PCR method based on different target genes for rapid detection of Bacillus cereus

To develop a rapid and sensitive real-time PCR method to differentiate emetic Bacillus cereus and non- emetic B.cereus based on different target genes.DNA was isolated by boiling method and used to test the specificity of primers by PCR.The rice was inoculated by emetic B.cereus for imitating contaminated food and detected by real- time PCR method.The results showed that primers were specific and high of amplification efficiency, the B.cereus could be quantified accurately by real-time PCR and the limit of detection was 101CFU /g in the contaminated rice.The novel developed real- time PCR method was specific, sensitive and accurate, which could be used for the rapid detection of B.cereus in food.