Construction of genetically engineering Escherichia coli for resveratrol production

LIANG Jing-long; GUO Li-qiong; SUN Ping; LIN Jun-fang; HE Yang

doi:10.13386/j.issn1002-0306.2013.23.091

LIANG Jing-long, GUO Li-qiong, SUN Ping, LIN Jun-fang, HE Yang. Construction of genetically engineering Escherichia coli for resveratrol production[J]. Science and Technology of Food Industry, 2013, (23): 138-142. DOI: 10.13386/j.issn1002-0306.2013.23.091

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Construction of genetically engineering Escherichia coli for resveratrol production

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To construction of expression plasmid pMD18- Vvrs- At4cl by touchdown overlap extension PCR and ligase connection.The constitutive vector containing a gap promoter (pGAP) of Escherichia coli and 4- coumaroyl:CoA ligase gene of Arabidopsis thalianna (At4CL) and resveratrol synthase gene of Vitis vinifera, (VvRS) , which transformed into host strain E.coli BW27784.Next, with fermentation and HPLC detection, created an recombination E.coli strain caple of bioproducing resveratrol by additionally supplemented with p-coumaric acid and resulted in a final titer of 4.51mg /L resveratrol.

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