PCR method in discriminating liquid malt extract and corn syrup

Widely-used food additives, liquid malt extract and corn syrup, were used as experimental materials.The improved CTAB, SDS and kit methods were used to extract DNA.The specific primers were designed based on the B1 hordein gene and corn starch branching enzyme gene.The results showed that the improved CTAB DNA extraction could get good quality DNA.When using the above primers for PCR, the product was single band and no non- specificity amplification.These results indicated the method could accurately distinguish liquid malt extract and corn syrup, which laid the foundation for application in direct detection and distinguishing the liquid malt extract and corn syrup.