Analysis of monosaccharide compositions of Nostoc Flagelliforme extracellular polysaccharide by pre-column derivation HPLC

The polysaccharide was precipitated by ethanol, and hydrolyzed into monosaccharides by trifluoroacetic (TFA) . The hydrolyzate was derivated with 1-phenyl-methyl-5-pyrazolone (PMP) and then the PMP derivates of monosaccharides were determined by RP-HPLC. The results showed that Nostoc Flagelliforme extracellular polysaccharide was composed of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and the molar ratio of the six monosaccharides were 0.71∶0.37∶0.44∶1.53∶1.03∶0.40. The established HPLC method was simple, fast and good reproducibility, and could be used for the determination of monosaccharide compositions of Nostoc Flagelliforme extracellular polysaccharide.