LI Xi-yue, GAO Zhe, CUI Can, AN Xiao-nan, WANG Rong-fang, CUI Tong. Study on structural identification and antioxidant activity of flavonoids from jujube leaves[J]. Science and Technology of Food Industry, 2015, (10): 135-138. DOI: 10.13386/j.issn1002-0306.2015.10.019
Citation: LI Xi-yue, GAO Zhe, CUI Can, AN Xiao-nan, WANG Rong-fang, CUI Tong. Study on structural identification and antioxidant activity of flavonoids from jujube leaves[J]. Science and Technology of Food Industry, 2015, (10): 135-138. DOI: 10.13386/j.issn1002-0306.2015.10.019

Study on structural identification and antioxidant activity of flavonoids from jujube leaves

More Information
  • Received Date: September 09, 2014
  • Five kind of flavonoids in the jujube leaves were obtained by the isolation with ethanol as the extraction reagent and subsequently purification of ethyl acetate fraction of the ethanol extract by a preparative HPLC, whose structures were verified by1 H NMR and13 C NMR. The five compounds were identified as quercetin-3-O-robinobioside (1) , quercetin-3-O-rutinoside (2) , quercetin-3-O-β-D-glucoside (3) , kaempferol-3-Orutinoside (4) and quercetin-3-O-α-L-arabinosyl- (1→2) -α-L-rhamnoside (5) , respectively. All compounds were evaluated for antioxidant capacity against ABTS+and DPPH radicals. Results revealed similar trends for the capability of the five flavonoids to scavenge the ABTS+and DPPH radicals. Among them, compound 3exhibited the highest activity and compound 1, 2, and 5 showed moderate activity, while compound 4 revealed lower antioxidant capacity.
  • [1]
    李淑子, 张本.大枣的化学和药理研究概况[J].中草药, 1983, 14 (10) :39-43.
    [2]
    Gao Q H, Wu C S, Wang M.The jujube (Ziziphus jujuba Mill.) fruit:a review of current knowledge of fruit composition and health benefits[J].J Agric Food Chem, 2013, 61 (14) :3351-3363.
    [3]
    车勇, 郑冰清, 滕勇荣, 等.酸枣叶化学成分的研究[J].中成药, 2012, 34 (4) :686-688.
    [4]
    Guo S, Duan J A, Tang Y P, et al.Simultaneous qualitative and quantitative analysis of triterpenic acids, saponins and flavonoids in the leaves of two Ziziphus species by HPLC-PDAMS/ELSD[J].J Pharm Biomed Anal, 2011, 56 (2) :264-270.
    [5]
    Zhang R, Chen J, Shi Q, et al.Quality control method for commercially available wild jujube leaf tea based on HPLC characteristic fingerprint analysis of flavonoid compounds[J].J Sep Sci, 2014, 37 (1-2) :45-52.
    [6]
    Zhang R, Chen J, Shi Q, et al.Phytochemical analysis of Chinese commercial available Ziziphus jujube leaf tea using high performance liquid chromatography-electrospray ionization-time of flight mass spectrometry[J].Food Res Int, 2014, 56:47-54.
    [7]
    曲泽洲, 王永惠, 刘孟军.酸枣的化学成分及其药理研究[J].河北农业大学学报, 1987, 10 (2) :60-66.
    [8]
    Re R, Pellegrini N, Proteggente A, et al.Antioxidant activity applying an improved ABTS+radical cation decolorization assay[J].Free Radical Biol Med, 1999, 26 (9) :1231-1237.
    [9]
    林恋竹, 赵谋明.反应时间对DPPH·法、ABTS+·法评价抗氧化性结果的影响[J].食品科学, 2010, 31 (5) :63-66.
    [10]
    何童森.山楂中红果酸GC-MS分析方法的建立及黄烷醇抗氧化作用研究[D].保定:河北农业大学, 2011.
    [11]
    Brand-williams W, Cuvelier M E, Berset C.Use of a free radical method to evaluate antioxidant activity[J].Lebensm-Wiss Technol, 1995, 28:25-30.
    [12]
    郝福玲, 方访, 凌铁军, 等.夹竹桃叶化学成分的研究[J].安徽农业大学学报, 2013, 40 (5) :795-801.
    [13]
    Deng S, Deng Z, Fan Y, et al.Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography[J].J Chromatogr B, 2009, 877 (24) :2487-2492.
    [14]
    Juan-Badaturuge M, Habtemariam S, Thomas M J K.Antioxidant compounds from a South Asian beverage and medicinal plant, Cassia auriculata[J].Food Chem, 2011, 125 (1) :221-225.
    [15]
    石心红, 王宇行, 孔令义.准噶尔大戟根中黄酮类成分的研究[J].中国药学杂志, 2006, 41 (20) :1538-1540.
    [16]
    Luo Y, Li X, He J, et al.Isolation, characterisation, and antioxidant activities of flavonoids from chufa (Eleocharis tuberosa) peels[J].Food Chem, 2014, 164:30-35.
    [17]
    Ammar R B, Bhouri W, Sghaier M B, et al.Antioxidant and free radical-scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L. (Rhamnaceae) :A structure-activity relationship study[J].Food Chem, 2009, 116 (1) :258-264.

Catalog

    Article Metrics

    Article views (106) PDF downloads (323) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return