Optimization of ultrasonic-assisted extraction process of colchicine from lily flower by response surface methodology

Lily flower as the research object, using the response surface analysis methodology the ultrasonic extraction technology of colchicine was optimazed. On the basis of single-factor tests, the effects of ethanol concentration, extraction duration, extraction temperature, and liquid-material ratio on colchicine content were explored. A regression model describing extraction of colchicine as a function of three variables was established with the Design-Expert software according to the Box-Behnken design principle and experimental data. The results showed that the optimal technologies were 60% ethanol as extraction solvent, extraction temperature50℃, material liquid ratio 1∶21 and extraction time 33 min. Under the optimum condition lily alkaloid extracting yield was 16.25μg/g.