Optimization of purifying linoleic acid from walnut oil with urea adduction fractionation

The technology of separating linoleic acid from walnut oil by urea adduction was optimized in the paper. On the basis of single factor experiments, the effects of adduction temperature (℃) , adduction time (h) , m1 (mixed fatty acid) ∶m2 (urea) , V (95% ethanol) ∶m2 (urea) on the purity and yield of linoleic acid were analyzed by response surface method. The results showed that the optimal parameters of the urea adduction technology were adduction temperature-10 ℃, adduction time 24 h, m1 ( mixed fatty acid) ∶ m2 ( urea) =0. 3 and V ( 95%ethanol) ∶m2 ( urea) =4. On this condition, the purity of linoleic acid could rise from original 52.1 % to above80.20% by initial urea adduction.