Study on rapid identification of Panax quinquefolius and Panax ginseng based on improved ERIC-PCR

WANG Rui; YANG Guo; WANG Hao-nan; LI Ming-hua; YAO Shi-xin

doi:10.13386/j.issn1002-0306.2015.20.008

WANG Rui, YANG Guo, WANG Hao-nan, LI Ming-hua, YAO Shi-xin. Study on rapid identification of Panax quinquefolius and Panax ginseng based on improved ERIC-PCR[J]. Science and Technology of Food Industry, 2015, (20): 80-82. DOI: 10.13386/j.issn1002-0306.2015.20.008

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Study on rapid identification of Panax quinquefolius and Panax ginseng based on improved ERIC-PCR

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Abstract

Studied on the rapid identification and quantification of PQ and PG using improved ERIC-PCR,and a pair of new primers(ER1 and EL2) was found. When the annealing temperature was 46.0 ℃,the genome DNA PCR results showed one specific 2.8 kb band for PQ while two bands of 2.8 kb and 3.4 kb repectively for PG.It also was possible to quantify PG in mixed PQ and PG sample by quantification of the 3.4 Kb band,and the systematic error was only 5.2% and RSD was 3.5%. The study showed that this method was rapid and accurate to identify PQ and PG and to quantify PG in mixed PQ and PG.

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Study on rapid identification of Panax quinquefolius and Panax ginseng based on improved ERIC-PCR

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