Expression of Euglena gracilis △9 fatty acid elongase gene in Yarrowa Lipolytica

The △9-fatty acid elongase was cloned from c DNA of the Euglena gracilis. The sequence from c DNA was ligated into p INA1297,yielding the recombinant p INA1297-△9E. Yarrowa Lipolytica Po1 f was transformed with p INA1297- △9E by electroporation method and the positive transformants YL△9E were obtained by YNBcasa plates. The growth rate of transgenic strains was much higher than that of wild strain in YPD medium.Analysis of gas chromatography/mass spectrometer(GC-MS) showed that eicosadienoic acid(EDA;C20∶2n-6)was detected and their percentage to total fatty acids in transgenic Yarrowa Lipolytica was 14.8%,which indicated that Euglena gracilis △9-fatty acid elongase gene was expressed in transgenic Yarrowa Lipolytica.