Study on mutation breeding of Bacillus amyloliquefaciens producing milk- clotting enzyme

GUAN Ming-ling; LI Xue-peng; YANG min; ZHANG Zhong-ming; ZHANG Wei-bing; SHI Xi-xong

doi:10.13386/j.issn1002-0306.2016.03.025

GUAN Ming-ling, LI Xue-peng, YANG min, ZHANG Zhong-ming, ZHANG Wei-bing, SHI Xi-xong. Study on mutation breeding of Bacillus amyloliquefaciens producing milk- clotting enzyme[J]. Science and Technology of Food Industry, 2016, (03): 156-159. DOI: 10.13386/j.issn1002-0306.2016.03.025

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Study on mutation breeding of Bacillus amyloliquefaciens producing milk- clotting enzyme

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Abstract

To obtain an industrial strain with high milk- clotting activity,Bacillus amyloliquefaciens producing milkclotting enzyme was mutagenized with ultraviolet and diethyl sulfate. On the basis of multiple mutation,mutant DES-6 was screened.The milk- clotting activity of L-6 reached 1419.7 SU / m L,which was 40.2% higher than that of the parent strain,while the proteolytic activity was reduced by 22.39%. The ratio of milk- clotting activity to proteolytic activity reached 125.64,which was 81.32% higher than that of the parent strain.The pass- generation test indicated that DES-6 had good genetic stability.

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