Optimization of submerge fermentation conditions of Agaricus Blazei Muril and dynamics analysis

The 3 L fermentation tank allocation fermentation research about Agaricus Blazei Muril batch fermentation dynamics was conducted on the basis of shaking flask conditions optimization,the metabolic regulation of Agaricus Blazei Murill bacteria growth,extracellular polysaccharide,substrate consumption were described based on Logistic and Luedekingpiret equation.The results showed that the optimum culture conditions were the stirring rate of 152 r / min,ventilation quantity of 0.58 m3/ h,inoculation amount of 6%,temperature of 24.6 ℃ and p H at 6.4.Under the optimized conditions,1.420 g /100 m L for dry weight of mycelium,6.955 g / L for the amount of exopolysaccharides were achieved.Bacteria growth and the activity of intracellular polysaccharide generated were synchronous,the dynamic mathematical model and model parameters of describing the batch fermentation process were obtained,at the same time,the validation comparition about experimental data and model parameters were conducted,the model calculation and the experimental results had a good fitting. The active polysaccharide was classified as coupling model and the model could be used to predict the fermentation process.