Study on enzymatic process of bovine bone for the peptides by response surface optimization

Bovine bone as the raw material,the enzymatic process for bone peptide was studied.Then on the basis of single- factor experiments,response surface analysis was adopted according to Box- Behnken center- united experimental design principle with hydrolysis degree and the rate of peptides as the responses. The optimum enzymolysis condition was obtained as follows: substrate concentration was 12%,hydrolysis p H was 7.5,enzyme concentration was 10500 U / g,hydrolysis temperature was 45 ℃,and hydrolysis time was 3.45 h,in this condition the rate of peptides and hydrolysis degree are 69.76% and 14.29%.The predicted values for optimization process conditions were in good agreement with experimental data.The results showed that enzymatic hydrolysis of bovine bone can produce peptides and provide experimental basis for the intensive processing of bone resources.