Multiplex LAMP method for the detection of pathogenic bacteria in eggs

Two strains of bacteria named X_1,X_2 were found to detect the pathogenic bacteria on eggs. Combined with 16 S r RNA molecule methods,testing the cloned plasmid sequence,blasting the sequence with NCBI in Gen Bank. The results showed that X_1 was Bacillus cereus NC7401(AP007209) and X_1 was Staphylococcus aureus(AP003088). According to the highly conservative gene nhe and nuc,two sets of primers were designed.The optimized LAMP reaction system contained Mg SO41.5 μL,each primer 2.0 μL and reaction temperature of60 ℃. The specificity was verified by using 9 strains of non-targeting bacteria and targeting bacteria X_1,X_2.The LAMP assay showed a sensitivity of 10 fg / μL. The novel multiple LAMP method reported here was characteristics of rapid detection, high specificity and excellent sensitivity, which could be applied in the detection of pathogenic bacteria.