MA Jing-fan, LIU Xi-ming, CHEN Xiao-hong. Antioxidant activity-guided fractionation of non-heading Chinese cabbage[J]. Science and Technology of Food Industry, 2016, (04): 132-137. DOI: 10.13386/j.issn1002-0306.2016.04.017
Citation: MA Jing-fan, LIU Xi-ming, CHEN Xiao-hong. Antioxidant activity-guided fractionation of non-heading Chinese cabbage[J]. Science and Technology of Food Industry, 2016, (04): 132-137. DOI: 10.13386/j.issn1002-0306.2016.04.017

Antioxidant activity-guided fractionation of non-heading Chinese cabbage

  • This study was carried out to explore the antioxidant activities of five extracts obtained from non-heading Chinese cabbage by using different polarity solvents, including petroleum ether, chloroform, ethyl acetate,n-butyl alcohol and water. The antioxidant activities of the five fractions were evaluated by the scavenging power of four free radicals,namely DPPH·,·OH,O2-· and ABTS+·. The relationship between the antioxidant activity and the content of total phenolics and total flavonoids were also analysed. The results showed that four fractions except water fraction had great antioxidant activity and the content of total phenolics and total flavonoids had a highly significant correlation with the antioxidant activity. The ethyl acetate fraction was the most effective fraction. The EC50 values based on DPPH·,·OH,O2-· and ABTS+· were(0.64±0.05),(1.17±0.12),(1.02±0.15) mg/m L and(0.78±0.07) mg/m L,respectively. The highest contents of total phenolics and total flavonoids were in the ethyl acetate fraction and their contents were(151.32±1.87) mg gallic acid equivalent/g DW and(32.97±0.56) mg rutin equivalent/g DW,respectively. Effect of the ethyl acetate fraction on anti-lipid peroxidation was investigated. The inhibition of the ethyl acetate fraction on hemolysis of mice erythrocytes and lipid perexidation of mice liver homogenate induced by H2O2 were 62.11% and 51.26% when the ethyl acetate fraction concentration was 10 mg/m L. The ethyl acetate fraction of non-heading Chinese cabbage,a strong ability to act as antioxidant,might be considered as a natural source of active compounds.
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