Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR

In order to research the difference of yak meat's quality in different regions,total RNA was extracted from 4-year-old male yak 's muscle samples of Datong,Jiulong,Henan,Diqing,Hongyuan,and then c DNA was generated under the action of reverse transcription polymerase,at last,the production of reserve transcription reaction was utilized to carry out RT-PCR and detect the expression levels of FABP gene. The results were that if the yak muscle sample of Diqing was taken as the control group and the 2~(-ΔΔCt)value of it was set to 1,the fluorescence quantitative 2~(-ΔΔCt)value of Datong,Jiulong,Henan,Hongyuan yak muscles were 4.18,2.94,9.95,10.73 respectively. So that the relative expression quantity of FABP gene in yak muscles could be obtained to evaluate yak meat's quality in different regions on molecular level. The yak meat's quality of Hongyuan and Henan was the best while that of Diqing was the worst.