Physicochemical properties and antioxidant activity of acid polysaccharide sjp-2 from Semen Juglandis

The polysaccharide SJP- 2 from Semen Juglandis was obtained by hot water extraction and alcohol precipitation,and purified using DEAE- Sepharose Fast Flow chromatography and Sephadex- G50 gel column chromatography.The physicochemical properties and antioxidant activity of SJP- 2 were determined. The result showed that SJP- 2 without proteins,was a lower dispersion polysaccharide with uniform molecular of 3.239 ×10~3g / mol,and the purity was 99.61%.The SJP-2 was acid polysaccharide determined by HPLC,and composed of arabinose,galactose,glucose,and galacturonic acid with a molar ratio of 1 ∶ 2.62 ∶ 4.45 ∶ 18.53. The IC₅₀ of DPPH scavenging activity,hydroxyl radical scavenging activity,and ABTS scavenging activity were 0.094,0.945,0.591 mg / m L,respectively. At 1 mg / m L,the reducing power was 1.158. The ORAC of SJP- 2 was( 1217.99 ±31.22) μmol TE / g.It was demonstrated that SJP-2 had higher antioxidant activity.