Study on unteraction mechanism of antiyeast substance produced by lactobacillus and the yeast DNA

Two strains of lactobacillus ALAC- 3 and ALAC- 4 being of strong antiyeast ability were studied. Taking Candida albicans as the indicator strain,the interaction of antiyeast substance produced by lactobacillus and yeast DNA was investigated by ultraviolet spectrometry and fluorescence spectrometry. The results showed that the ultraviolet absorption value was decreased significantly and hypochromism were observed after adding anti-bacterial substances.With the extension of time,the ultraviolet absorption value was increased and hyperchromism were observed. The fluorescence intensity was decreased after adding anti- bacterial substances. When anti-bacterial substances and EB- DNA was reacted with each other,the fluorescence intensity was decreased significantly. And the fluorescence intensity was increased after EB and anti- bacterial- DNA was reacted. It indicated that there was the competition of anti- bacterial- DNA and EB- DNA in the solution system.