Effect of overexpression of glucose dehydrogenase on hydroxyfatty acids production of Escherichia coli

WANG Xiang- wei; XING Xiang; MA Qing- lin

doi:10.13386/j.issn1002-0306.2016.05.023

WANG Xiang- wei, XING Xiang, MA Qing- lin. Effect of overexpression of glucose dehydrogenase on hydroxyfatty acids production of Escherichia coli[J]. Science and Technology of Food Industry, 2016, (05): 163-166. DOI: 10.13386/j.issn1002-0306.2016.05.023

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Effect of overexpression of glucose dehydrogenase on hydroxyfatty acids production of Escherichia coli

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Abstract

A metabolic pathway to synthesize hydroxyfatty acids( HFAs) from glucose was constructed. But the reducibility( NADPH) imbalance hampers its application. In order to increase the yield of HFAs,the glucose dehydrogenase( GDH) from Bacillus megaterium was cloned and a NADPH and HFAs co- product strains was built to solve the reducibility imbalance in cell. Under shake- flask conditions,this recombinant strain produced173.9 mg / L HFAs.The research laid the foundation in industrial applications for obtaining engineered Escherichia coli to produce HFAs with high production.

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Effect of overexpression of glucose dehydrogenase on hydroxyfatty acids production of Escherichia coli

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