YUE Wei, LI Gang. Extraction of total flavonoids from leaves of Indocalamus using inner ebullition method[J]. Science and Technology of Food Industry, 2016, (05): 263-267. DOI: 10.13386/j.issn1002-0306.2016.05.043
Citation: YUE Wei, LI Gang. Extraction of total flavonoids from leaves of Indocalamus using inner ebullition method[J]. Science and Technology of Food Industry, 2016, (05): 263-267. DOI: 10.13386/j.issn1002-0306.2016.05.043

Extraction of total flavonoids from leaves of Indocalamus using inner ebullition method

  • Objective: To explore the optimum extraction process of total flavonoids from leaves of Indocalamus by inner ebullition method. Methods: Through single- factor experiments of desorption / extraction process and the orthogonal experiments,the optimal extract conditions of the total flavonoids from leaves of Indocalamus by using inner ebullition method was studied. The method was also compared with reflux method,ultrasonic method and microwave method.Results: The optimal desorption conditions were as follows: solid- liquid ratio was 1 ∶ 3,ethanol concentration was 90%,the resolution time was 25 min. The optimal extraction conditions were: solid- liquid ratio was 1∶ 30,the temperature was 85 ℃,ethanol concentration was 30%,the extraction time was 5 min.The yield of total flavonoids was 2.05%,the content of isoorientin and orientin was 1.656,0.454 mg·g-1.Comparing with reflux method,ultrasonic method and microwave method,the inner ebullition method had no significant difference of yield of flavonoids,but lesser amount of ethanol and cheaper cost.
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