Analysis of volatile compounds in Cordyceps cicadae and submergedly cultured mycelia of Isaria cicadae

In order to determine the volatile compounds in Cordyceps cicadae and submergedly cultured mycelia of Isaria cicadae,the strain GZU1205241 was identified by the sequence analysis of a r DNAITS fragment and the traditional identification methods(morphological characteristics of the colony and sporulation structure).Then the volatile compounds of wild Cordyceps cicadae mycelium and fermentation volatile compounds were extracted by headspace solid phase microextraction(HS-SPME) and steam distillation and determined by gas chromatography and mass spectrometry. The results showed that the strain GZU1205241 was Isaria cicadae.Cordyceps cicadae and mycelia of Isaria cicadae,respectively,contained 56 and 58 volatile compounds.Hereinto,25 compounds in both wild and mycelia. The main volatile compounds of Cordyceps cicadae mainly were acids,and the relative content of oleic acid,hexadecanoic acid and linoleic acid was 45.09%,21.18% and9.44%,respectively. The main volatile compounds of fermented mycelia were acids,and the relative content of palmitic acid,linoleic acid and oleic acid was 38.44%,18.58% and 12.83%,respectively. The volatile composition and relative content of wild Cordyceps Cicadae and fermented mycelia obtained by steam distillation and headspace solid phase micro extraction had obvious differences. The volatile composition were more by water vapor distillation. Acid were the main volatile compounds of Cordyceps cicadae and fermented mycelia obtained by steam distillation and headspace solid phase micro extraction.