Preparation and properties of cross-linked enzyme aggregates of cattle pancreatic lipase

In this study,cross-linked enzyme aggregates(CLEAs) of cattle pancreatic lipase(CPL-CLEAs) was prepared by cross-linked enzyme aggregates technology,the preparation conditions,catalysis properties,and dynamics parameters of CPL-CLEAs were investigated. The results showed that the optimum conditions of the preparation of CPL- CLEAs were as followed : final saturability of the ammonium sulfate was 80 %, final concentration of glutaraldehyde was 1. 5 %, and corss- link time was 1 h. Under this condition, the highest activity recovery reached 90%. In addition,compared to free lipase,the optimum temperature of CPL-CLEAs was 60 ℃,which was increased about 10 ℃. The thermal stability,operational stability,and storage stablility of CPL-CLEAs were significantly enhanced compared with free lipase. Furthermore,the CPL-CLEAs still retained 43% of its initial activity after consecutive 7 cycles.