Optimization of growth of lactic acid bacteria in synbiotics koumiss by central composite design

In order to study the effects of different oligosaccharides on the growth of lactic acid bacteria in synbiotics koumiss,three oligosaccharides for promoting the growth of lactic acid bacteria were screened via single factor experiment,and the optimal additions of three substrates were confirmed by central composite design and response surface analysis. The results indicated that the optimal additions of three oligosaccharides were 1.30%(W/V) of galacto-oligosaccharides,0.80%(W/V) of isomaltooligosaccharide and 0.40%(W/V) of xylooligosaccharides. Under the optimal conditions,the viable counts of lactic acid bacteria could reach up to(5.97±0.16)×10~8 CFU/m L,which revealed an increase by 70.57%.