Study on virulence genes distribution and molecular subtyping of Vibrio parahaemolyticus from different food resources

The distribution of virulence genes、serogroup and molecular subtyping of Vibrio parahaemlyticus isolated from different resources of food were analysed,which contribute to provide scientific evidence for surveillance and source tracking. Forty strains of Vibrio parahaemolyticus genomes were extracted,VcrD1,Vp1680,Vop P,VcrD2 were amplified by PCR and tdh,trh,tlh genes were detected by Real-time fluorescent PCR. All of strains were subtyped by pulsed-field gel electrophoresis and serotyped. PFGE patterns were clustered by Bionumerics software. Virulence genes tdh,trh,Vop P and Vcr D2 were not detected,genes of tlh,VcrD1 and Vp1680 were all positive detected. Sixteen serotypes were observed,the serotyping of 9 isolates failed. The major were O₂∶K₂₈ and O₅∶K₁₇. No predominant serotype was detected. Thirty-nine PFGE patterns were detected and no predominant pattern. No virulence genes were detected. The isolates from food resource showed diversified serotype feature and far genetic distance.