Optimization of extraction conditions of polysaccharides from Campanumoea javanica Blume by response surface methodology and evaluation of its neurotrophic activities

Extraction optimization, purification and physicochemical properties of polysaccharides from Campanumoea javanica Blume( CJP) were investigated. The neurotrophic activity of polysaccharides from C. javanica Blume were investigated by PC12 cell assay. Single factor experiment and response surface methodology(RSM) were used to optimize the extraction conditions of CJP. As a result,the optimal conditions were as follows : extraction temperature, 67 ℃, extraction time, 52 min, and ratio of water to raw material,17 ∶ 1 m L / g. The crude CJP were purified by DEAE- Cellulose 52 chromatography and Sephadex G- 100 chromatography to afford CJPH and CJPN. The content of total sugar,protein,uronic acid and sulfate were analyzed by chemical analysis methods. HPLC analysis showed that CJPH and CJPN were homogeneous polysaccharide,the molecular weights of CJPH and CJPN were 3923 u and 7215 u,respectively. Monosaccharide composition analysis was acted by using the GC method. Infrared spectroscopy analysis indicated that CJPH and CJPN showed typical peaks of polysaccharide. The assay of PC12 cell showed that CJPH and CJPN had neurotrophic activity. Studies had shown that CJPH and CJPN could obviously repairing nerve cell damage,they had certain development value.