Catalytic synthesis of DHA-PC with high DHA contents by phospholipase A2 in supercritical CO2 system

With the raw material of euphausia superba powder, the purity of the refining phosphatidylcholine gained by ethyl alcohol extraction and separation and purification reached 90.8%, then the refining phosphatidylcholine was used as raw material and phospholipase A₂ was used to catalyze the fatty acid hydrolysis of Sn-2 position of phosphatidylcholine in supercritical CO₂ system to make the fish oil DHA to Sn-2 position and form the DHA-PC with high DHA contents.Meanwhile, the effects of different parameters on the reaction were surveyed.The results showed that, the best additive amount of immobilization phospholipase A₂ was 15% ( occupied the total substratio) , the best enzymolysis was 6 h, the best enzymolysis temperature was 55 ℃, the best enzymolysis pressure was 12 MPa, respectively.Under such condition, the DHA binding ratio of euphausia superba phosphatidylcholine could reach 62.3 mol%. And it could be concluded that immobilization phospholipase A₂ is highly viable for phosphatidylcholine modification to prepare DHA-PC.