Extraction and purification of total saponins from quinoa bran

Solvent reflux method was used for saponins extraction and the responsive surface methodology was used to optimize the related parameters.Adsorption rate and desorption rate were used as indicators for screening of proper resin for saponins purification and determining of purification techniques. The obtained optimal extraction technique with a ratio ( bran to 72%ethanol) of 1∶ 20.8, carried out at 72 ℃ for 147 min, and the obtaining rate of saponins reached 1.685%. Compared with HPD600, S-8 and AB-8, the resin D101 showed the highest absorption rate and the highest desorption rate, so it was used for later purification studies. The results suggested that the highest absorption rate for D101 was obtained with a sample concentration of 4 mg/m L, the loading sample was adjusted to p H6 and loading at a velocity of 2 BV/h.The highest desorption rate was obtained when elute with 90% ethanol at a speed of 2 BV/h. The desorption fraction was condensed and freezing dried, the purity of resulted saponins powder reached 43.6%. The above results will facilitate the high-value application of quinoa bran and the related research work of quinoa saponins.