Residue determination of forchlorfenuron in soil by solid-phase extraction and ultra performance liquid chromatography

The forchlorfenuron residues in soil were determined quickly by solid-phase extraction and ultra performance liquid chromatography ( SPE-UPLC) in this paper. And this paper compared to determine the appropriate pre-treatment methods, optimized the extraction method and purification conditions.The optimal conditions were obtained that the sample was extracted with acetonitrile for 30 min by ultrasonic method, centrifuged in 5000 r/min and cleaned up with the PSA column.Forchlorfenuron was determined by ultra performance liquid chromatography using acetonitrile-water ( 65 ∶ 35) as mobile phase.The developed method showed a good linear relationship in the range of 0.05 ~ 5.0 μg/m L for forchlorfenuron, with correlation coefficient of 0.9999.The limit of quantitation for forchlorfenuron was 0.025 mg/kg. The average recovery rates were78.34% ¹02.4%, with relative standard deviation ( RSD) ranging from 3.24% to 6.65%.The method is fast, highly sensitivity, repeatable and suitable for the determination of forchlorfenuron residues in soil.