WANG Yang, WANG Jing-ru, BAI Dong-qing, DAN Qing-hua, YU Bo, YU Zheng-hao, LUO Yun-long. Synergistic Inhibitory Effects of Nisin and L-Lactic Acid against Aeromonas hydrophila[J]. Science and Technology of Food Industry, 2020, 41(16): 81-87. DOI: 10.13386/j.issn1002-0306.2020.16.014
Citation: WANG Yang, WANG Jing-ru, BAI Dong-qing, DAN Qing-hua, YU Bo, YU Zheng-hao, LUO Yun-long. Synergistic Inhibitory Effects of Nisin and L-Lactic Acid against Aeromonas hydrophila[J]. Science and Technology of Food Industry, 2020, 41(16): 81-87. DOI: 10.13386/j.issn1002-0306.2020.16.014

Synergistic Inhibitory Effects of Nisin and L-Lactic Acid against Aeromonas hydrophila

  • To investigate the synergistic inhibitory effect and mechanism of nisin and L-lactic acid against Aeromonas hydrophila,turbidity,plate count method,membrane electromotive force probe were used to detect the influence of nisin and L-lactic acid on growth,survival,biofilm formation,and membrane integrity of Aeromonas hydrophila(ATCC 35654,CICC 10500). The results showed that nisin and low concentration(3.5 mmol/L)of L-lactic acid synergistically inhibited the growth of two strains of Aeromonas hydrophila. And the inhibitory effect strongthened with the increasing of nisin concentration. The growth parameters modeled with a Gomptz equation showed that nisin and L-lactic acid synergistically prolonged the growth lag phase of Aeromonas hydrophila,the maximum specific growth rate decreased,and the maximum viable count of bacteria at stationary phase also decreased. Besides,nisin and L-lactic acid had a synergistic lethal effect on the indicator bacteria. Nisin and L-lactic acid treated for 12 h reduced the viable count of Aeromonas hydrophila. Results of the mechanism indicated that low concentration of L-lactic acid released the outer membrane LPS from the bacterial cells,and nisin further dissipated the membrane potential. The results would provide a theoretical basis for the use of nisin and L-lactic acid or their producing strains in control of Aeromonas hydrophila in aquatic products.
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