LIU Hai-bin, ZHANG Hai-xin, SONG Teng-fei, LIU Cong, NIE Fu-lei. Rapid Detection of Duck Derived Components in Mutton and Mutton Products by Loop Mediated Isothermal Amplification Combined with Immunochromatographic Test Strip[J]. Science and Technology of Food Industry, 2020, 41(16): 238-242,251. DOI: 10.13386/j.issn1002-0306.2020.16.038
Citation: LIU Hai-bin, ZHANG Hai-xin, SONG Teng-fei, LIU Cong, NIE Fu-lei. Rapid Detection of Duck Derived Components in Mutton and Mutton Products by Loop Mediated Isothermal Amplification Combined with Immunochromatographic Test Strip[J]. Science and Technology of Food Industry, 2020, 41(16): 238-242,251. DOI: 10.13386/j.issn1002-0306.2020.16.038

Rapid Detection of Duck Derived Components in Mutton and Mutton Products by Loop Mediated Isothermal Amplification Combined with Immunochromatographic Test Strip

  • In order to realize the rapid detection of adulteration in meat and meat products,this paper combined loop mediated isothermal amplification(LAMP)with immunochromatographic test strip(ICTS)to establish a specific and sensitive method for detecting duck meat in mutton and its products. LAMP primers were designed according to six specific regions of duck specific cytochrome b(cytb)gene. Two inner primers,FIP and BIP,were labeled with biotin and digoxin respectively,and amplified at 65℃ for 30 min to produce double-strand DNA produces labeled with biotin and digoxin. The products(10 μL)was mixed with 90 μL loading buffer(PBS pH=7.4),and then the ICTS was used to realize visual detection. The LAMP-ICTS method established in this study could specifically detect duck ingredients within 40 min,and there was no cross reaction with other meats. At the same time,the detection limit of the target in meat products could reach 0.01%(w/w). The test results of commercial samples showed that there were duck-derived components in the samples of mutton slices and mutton kettles,and the test results were consistent with the industry standard method(PCR-electrophoresis). Therefore,LAMP-ICTS method had the advantages of high sensitivity,good specificity,rapid detection,low dependence on instruments,and was suitable for the rapid detection of meat adulteration in the laboratories of both regulatory agencies and commercial services.
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