Intervention Effects of Chicory,Pueraria and Mulberry Leaf Formula on Reducing Uric Acid and Gouty Arthritis in Mice with High Uric Acid and Arthritis

objective:In order to investigate the potential uric acid lowering and anti-gout effect and mechanism of the formulation of chicory,pueraria and mulberry leaves. Methods:50 KM male mice were randomly divided into normal,model,allopurinol-positive(5 mg·kg^-1),high-dose(2.67 g·kg^-1)and low-dose(1.33 g·kg^-1)groups,with 10 mice in each group. Potassium oxonate was injected intraperitoneally at a dose of 0.1 mL/10 g to make the model mice. One hour later,the formula was gavage at a dose of 0.1 mL/10 g. On day 9 of the model,sodium urate crystal suspension(25 mg·mL^-1)was used to induce gout arthritis. Inflammatory factors,uric acid(UA),xanthine oxidase(XOD)as well as liver and kidney function indexes in serum were detected,and the expression of urate transporter(URAT1)was detected by Western Blot. Results:Compared with the model group,both the high and low dose groups could reduce the index of joint inflammation in mice,and the level of joint infrared temperature,serum UA,TNF-α,CRE,BUN,AST and ALT were significantly decreased(P<0.05 or P<0.01).The degree of joint swelling and IL-1β level were decreased in the high-dose group(P<0.05),and the XOD level was significantly decreased(P<0.01). The degree of joint swelling was decreased and the morphology of liver and kidney tissues was improved. And the expression of URAT1 protein in kidney tissues was decreased(P<0.05). Conclusion:The formulations of chicory,pueraria and mulberry leaves possessed the functions of lowering uric acid and anti-gout,the mechanism of which may be related to improving liver and kidney injury,lowering serum uric acid and reducing the expression of URAT1 protein in kidney tissues.