Prediction of Linear Epitope of Tropomyosin in Macrobrachium nipponense

The linear epitopes of tropomyosin in M.nipponense were predicted,which provided the basis for the identification and detection of related food allergies and the targets for the reduction of allergenicity based on the epitope. In this study,the B-cell linearepitopes of tropomyosin in M. nipponense were predicted by DNAStar and online network servers including SOPMA,BepiPred 1.0 Server and ABCpred. The T-cell epitopes were predicted by SYFPEITHI,NetMHCII 2.3 Server and NetMHCIIpan 3.2 Server. Based on the above comprehensive analysis,the probable B-cell linear epitopes might contain ²¹RADTLEQQNKEANN³⁴,³⁷EKTEEEIRTTQKKMQQ⁵²,⁷¹LEEKEKA⁷⁷,⁹⁹LERSEERLN¹⁰⁷,¹¹⁹AADESER¹²⁵,¹³⁴SLSDEER¹⁴⁰,¹⁵⁸ADRKYDE¹⁶⁴,¹⁷⁷ERAEERAETG¹⁸⁶,²¹⁰SEEKANQREEAYKE²²³,²⁶²NEKEKYK²⁶⁸ and the possible T-cell epitopes were ⁸²EGEVAALNRRIQLL⁹⁵,¹⁰⁵RLNTATTKLAEAS¹¹⁷,¹⁶⁵VARKLAMVEADLE¹⁷⁷,¹⁹⁵EELRVVGNNLKSLE²⁰⁸,²²²KEQIKTLTNKLKAA²³⁵. This research can provide a more accurate target for further study on M. nipponense allergen.