Effect of Water Extract of Amaranthus retroflexus on Blood Lipid on Mice Induced by High Fat Diet

Objective:To study the effect of water extract of Amaranthus retroflexus on hyperlipidemia mice. Methods:The mice model of hyperlipidemia was established by high fat diet(HFD). The mice were randomly divided into five groups:Control group(Con),model group(Mod),lovastatin group(Lovastatin,10 mg/kg/d),low dose of water extract of Amaranthus retroflexus group(AR-L,430 mg/kg/d)and high dose of water extract of Amaranthus retroflexus group(AR-H,740 mg/kg/d). The control group was fed with basic feed,the rest groups were fed with high-fat feed,and the drug was given by gavage according to the dosage. After eight weeks,the contents of serum total cholesterol(TC),triglycerides(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),superoxide dismutase(SOD),malondialdehyde(MDA),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)levels in mice were determined.Mice liver histopathology was observed using hematoxylin and eosin(HE)staining.Results:Compared with the control group,the mice liver index extremely significantly increased(P<0.01)in the model group.The levels of mice serum TC,TG,LDL-C,ALT,AST and MDA significantly elevated and HDL-C,SOD extremely significantly reduced(P<0.01). Compared with the model group,the mice liver index extremely significantly increased(P<0.01)in the AR-H group. The levels of mice serum TC,TG and ALT extremely significantly reduced and SOD,HDL-C extremely significantly elevated(P<0.01),and the levels of mice serum MDA,AST significantly reduced(P<0.05). Compared with the model group,the mice liver index significantly increased(P<0.05)in the AR-L group. The levels of mice serum TC,TG and LDL-C significantly reduced(P<0.05),and the levels of mice serum SOD and HDL-C significantly elevated(P<0.01)and ALT extremely significantly reduced(P<0.01),and there were no significant difference in the levels of MDA and AST between the model group and AR-L group(P>0.05).The results of HE staining showed that the water extract of Amaranthus retroflexus could significantly reduce the lipid deposition and fatty degeneration of liver cells. Conclusion:The research shows that the water extract of Amaranthus retroflexus can significantly reduce the blood lipid level of hyperlipidemia model mice and improve their liver function.