Research Progress of Signal Amplification Strategies in Immunochromatographic Test Strip

Immunochromatographic Test Strip (ICTS), a detection technology based on capillary column chromatography and specific immune interaction between antibody and antigen, is widely utilized in the field of rapid detection of food safety detection due to its cost-effectiveness, easy operation, non-professional personnel, short assay time, high specificity and naked eye visualization. Conventional spherical gold nanoparticles-based ICTS is the most common detection method. However, the conventional ICTS can help achieve simple qualitative or semiquantitative analysis, its low sensitivity is an obstacle to meet the current detection requirements. Therefore, there has been a growing interest focusing on the signal amplification method to improve the sensitivity of ICTS devices. This paper summarizes signal amplification strategies to enhance the sensitivity of ICTS and proposes the future perspectives, which provides technical references for the development of rapid detection methods for food safety.