Anti-melanogenesis and Anti-wrinkle Effects of the Fucoidan Isolated from Turbinaria ornata

Objective: Investigating the in vitro anti-melanogenesis and anti-wrinkle effects of the fucoidan TO-F10 isolated from Turbinaria ornata. Methods: The colorimetric method was utilized to evaluate the inhibitory effects of TO-F10 at different concentrations on tyrosinase and collagenase in this study. The methyl thiazolyl tetrazolium (MTT) assay, DOPA oxidation assay, 2',7'-dichlorofluorescin diacetate (DCFH2-DA) assay, and enzyme-linked immunosorbent assay (ELISA) were used to examine the effects of TO-F10 on the cell viability, melanin content and tyrosinase activity in α-melanocyte-stimulating hormone (α-MSH)-induced mouse melanoma cells (B16F10), as well as its impact on the reactive oxygen species (ROS) level, the collagen content, matrix metalloproteinase (MMP) expression, and pro-inflammatory cytokine production in ultraviolet B (UVB)-induced human dermal fibroblasts (HDF). Results: At a concentration of 100 µg/mL, TO-F10 inhibited tyrosinase and collagenase by 26.98%±1.76% and 37.60%±0.60%, respectively. The increase in melanin content and decrease in tyrosinase activity in α-MSH-induced B16F10 cells were significantly reversed by TO-F10 treatment (P<0.01). UVB-exposed HDF cells were protected by TO-F10 through the inhibition of oxidative stress, collagen degradation, and inflammation. However, it significantly increased in cell viability were observed only at 50 and 100 µg/mL (P<0.05). Conclusion: TO-F10 was shown to possess anti-melanogenesis and anti-wrinkle properties, with its applicability in the functional food industry being indicated.