Effect of the Enrichment of Gamma-Aminobutyric Acid in Germinating Purple Kidney Bean by the Cold Atmospheric Pressure Plasma Combined with V_B6 and V_C

This study aimed to investigate the enrichment effect of cold atmospheric pressure plasma (CAPP) combined with V_B6 and V_C on gamma-aminobutyric acid (GABA) content enrichment of purple kidney bean germination. The purple kidney beans were used as raw materials. And the effects of different concentrations of V_B6 and V_C on the GABA enrichment of germinated beans treated with cold atmospheric pressure plasma as well as the activities of related metabolic enzymes were investigated. The results demonstrated that the method of cold atmospheric pressure plasma treatment with V_B6 and V_C promoted the enrichment of GABA in sprouted purple kidney beans. The GABA enrichment content reached 10.05±0.93 mg/g at 72 h of germination when treated with the CAPP combined with 0.25 mg/mL V_C. The GABA enrichment content reached 10.09±0.06 mg/g at 72 h germination when treated with CAPP combined with 0.5 mg/mL V_B6. Analysis of enzyme activities of germinated purple kidney beans at 72 h revealed that CAPP, V_B6 and V_C treatments enhanced glutamate decarboxylase (GAD) activity while partially suppressing polyamine oxidase (PAO) activity. The combination of CAPP with V_B6 and CAPP with V_C for the germination of purple kidney beans both enriched GABA by enhancing the activity of GAD and suppressing the activity of GABA transaminase (GABA-T). The result showed that the treatment condition had a promotion effect on the enrichment of GABA in the germination of kidney beans. And the result established a theoretical foundation for the production of high GABA rich food.