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中国精品科技期刊2020
李佳南, 王静, 张慧娟, 李莹莹, 刘英丽, 庞小一, 姜国庆. 大米蛋白抗氧化肽的活性以及组成鉴定研究[J]. 食品工业科技, 2013, (23): 53-57. DOI: 10.13386/j.issn1002-0306.2013.23.080
引用本文: 李佳南, 王静, 张慧娟, 李莹莹, 刘英丽, 庞小一, 姜国庆. 大米蛋白抗氧化肽的活性以及组成鉴定研究[J]. 食品工业科技, 2013, (23): 53-57. DOI: 10.13386/j.issn1002-0306.2013.23.080
LI Jia-nan, WANG Jing, ZHANG Hui-juan, LI Ying-ying, LIU Ying-li, PANG Xiao-yi, JIANG Guo-qing. Enzymatic preparation of antioxidant peptides from rice protein and identification of antioxidant peptides[J]. Science and Technology of Food Industry, 2013, (23): 53-57. DOI: 10.13386/j.issn1002-0306.2013.23.080
Citation: LI Jia-nan, WANG Jing, ZHANG Hui-juan, LI Ying-ying, LIU Ying-li, PANG Xiao-yi, JIANG Guo-qing. Enzymatic preparation of antioxidant peptides from rice protein and identification of antioxidant peptides[J]. Science and Technology of Food Industry, 2013, (23): 53-57. DOI: 10.13386/j.issn1002-0306.2013.23.080

大米蛋白抗氧化肽的活性以及组成鉴定研究

Enzymatic preparation of antioxidant peptides from rice protein and identification of antioxidant peptides

  • 摘要: 采用碱性蛋白酶酶解大米蛋白制备大米蛋白抗氧化肽。研究表明大米蛋白酶解产物具有螯合金属铁离子、清除H2O2、羟自由基、DPPH自由基、抑制亚油酸自然氧化的作用。通过应用高解析离子淌度质谱(HD-MS)分析鉴定米蛋白抗氧化肽的序列主要为Met-Pro-Pro-Ser-Ser-Pro-His(376.68u),Leu-Ala-Gly-Pro-Lys-Phe-Ala-Leu(408.75u)和Met-Pro-Arg-His-Asp-Pro-Gln(440.70u)。 

     

    Abstract: This antioxidant peptides prepared from rice protein hydrolyzed by Alcalase were characterized for their antioxidant properties in vitro reflected by the abilities to scavenge hydrogen peroxide as well as hydroxyl and DPPH free radicals, to chelate iron ions, and to inhibit autooxidation of linoleic acid.The antioxidant peptides were identified to be Met-Pro-Pro-Ser-Ser- Pro- His (376.68u) , Leu- Ala- Gly- Pro- Lys- Phe- Ala- Leu (408.75u) , and Met-Pro-Arg-His-Asp-Pro-Gln (440.70u) by High Definition Mass Spectrometry (HD-MS) .

     

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