Abstract: The hepatoprotective effect of mixture from water extracts of Hovenia duleis, Morus alba, and Canarium album (mass ratio of 2.2∶3.8∶2.8) on mice was studied. Mice were reared with the mixture through stomach perfusion for 30 day, and mice were treated with white wine by gastric perfusion. The contents of GSH, MDA, TG and the enzyme activity of ADH were determined to evaluate the mixture. The results showed that high, middle, low dose groups of the mixture could significantly increase GSH (p<0.01) in liver of mice, and decrease MDA (p<0.01) . However, high, middle dose groups could significantly decrease TG (p<0.01) . High, middle, low dose groups could significantly activated ADH (p <0.01) in liver of mice. The mixture performed the hepatoprotective effect on mice. The work provided a strong data support for the hepatoprotective effect of the mixture. In addition, all of three plant materials in the mixture are the medicinal and edible plant, which could be developed as hepatoprotective health food.