LI Yin-qing, YILI Abulimit, AISA Haji-akber. Purification of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica[J]. Science and Technology of Food Industry, 2014, (13): 236-240. doi: 10.13386/j.issn1002-0306.2014.13.042
Citation:
LI Yin-qing, YILI Abulimit, AISA Haji-akber. Purification of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica[J]. Science and Technology of Food Industry, 2014, (13): 236-240. doi: 10.13386/j.issn1002-0306.2014.13.042
LI Yin-qing, YILI Abulimit, AISA Haji-akber. Purification of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica[J]. Science and Technology of Food Industry, 2014, (13): 236-240. doi: 10.13386/j.issn1002-0306.2014.13.042
Citation:
LI Yin-qing, YILI Abulimit, AISA Haji-akber. Purification of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica[J]. Science and Technology of Food Industry, 2014, (13): 236-240. doi: 10.13386/j.issn1002-0306.2014.13.042
Key Laboratory of Plant Resources and Chemistry of Arid Zone, State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences
Objective: To establish the purification craft of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica. Method: The adsorption and desoption ability of the total triterpenoids and phenolics were carried out to select the appropriate marcoporous resin.Then, the dynamic adsorption and desoption experiments were used to study the appropriate purification craft.Result: AB-8 resin was better than other resins.The optimum purification conditions: sample solution concentration 0.067g /mL, 910BV sample solution in pH6, flow rate 2BV/h, Elution solvent 70%, ethanol washing 3.5BV.Under these optimum conditions, the purified product contained total triterpenoids 49.3% and total phenolic 36.1%, the total effective fraction content was 85.4% 。
Lima C F, Ferreria M F, Wilson C P.Phenolic compounds protect HepG2 cells from oxidative damage:Relevance of glutathione levels[J].Life Sciences, 2006, 79 (21) :2056-2068.
[6]
Liu J.Oleanolic acid and ursolic acid:Research perspectives[J].Journal of Ethnopharmacology, 2005, 100 (1-2) :92-94.