Issue 04
Turn off MathJax
Article Contents
CHENG Chao, ZHANG Hong-hai, SHENG Wen-jun, HAN Shun-yu, WANG Jing. Extraction of β-Glucan from waste wine yeast by multiple cell-wall-broken technologies[J]. Science and Technology of Food Industry, 2016, (04): 111-116. doi: 10.13386/j.issn1002-0306.2016.04.013
Citation: CHENG Chao, ZHANG Hong-hai, SHENG Wen-jun, HAN Shun-yu, WANG Jing. Extraction of β-Glucan from waste wine yeast by multiple cell-wall-broken technologies[J]. Science and Technology of Food Industry, 2016, (04): 111-116. doi: 10.13386/j.issn1002-0306.2016.04.013

Extraction of β-Glucan from waste wine yeast by multiple cell-wall-broken technologies

doi: 10.13386/j.issn1002-0306.2016.04.013
  • Received Date: 2015-07-27
  • The cell wall of wine yeast was broken by high pressure homogenization and freezing-thawing methods collaboratively. Combined with enzymatic hydrolysis of compound protease and lipase,the effect of multiple cell-wall-broken technologies on purity of β-Glucan was studied. On the basis of single factor experiment,the Box-Behnken design was adopted. The concentration of yeast,homogeneous time and freezing-thawing water content were factors,and the purity of β-Glucan was response value,the extraction process of β-Glucan from waste wine yeast was optimized. The results showed that the optimal extraction parameters were obtained as follows:homogeneous pressure 70 MPa,concentration of yeast 13%,homogeneous time 34 min and freezingthawing water content 25%,while extraction purity of β-Glucan content was 91.69% and yield was 13.23%under these conditions. The process provided a reference basis for the development and utilization of Glucan from yeast.
  • loading
  • [1]
    高洁.酵母β-葡聚糖制备、改性增溶和溶液构象研究[D].北京:中国农业科学院,2013.
    [2]
    Graubaum H J,Busch R,Stier H,et al.A doubleblind randomized,placebo-controlled nutritional study using an insoluble yeastβ-glucan to improve the immune defense system[J].Food and Nutrition Sciences,2012,3:738-746.
    [3]
    Eiboshy M E,Eiashram A M,Abdelhamid F M,et al.Immunomodulatory effect of dietary Saccharomyces cerevisiae,β-glucan and laminaran in mercuric chloride treated Nile tilapia(Oreochromisniloticus)andexperimentally infected with Aeromonashydrophila[J].Fish&Shellfish Immunology,2010,28:802-808.
    [4]
    Kousik M,Surajit S,Sunil K B,et al.An immunostimulating water insolubleβ-glucan of an edible hybrid mushroom:Isolation and characterization[J].Fitoterapia,2013,84:15-21.
    [5]
    杜娜,杨雪山,韩舜愈,等.超声波辅助酶法分离提取葡萄酒泥酵母SOD工艺条件的优化[J].食品科学,2014,35(2):87-90.
    [6]
    李双石,冀振红,李渤,等.葡萄酒废酵母胞壁多糖提取工艺的优化研究[J].酿酒科技,2014(12):85-87.
    [7]
    刘晓永.酿酒酵母β-D-葡聚糖制备、构象及免疫功效研究[D].无锡:江南大学,2007.
    [8]
    郭卫芸,杜冰,袁根良,等.反复冻融法破壁啤酒废酵母的研究[J].酿酒科技,2009(3):103-105.
    [9]
    Chema B,Fabienne F,Guilhem J,et al.Enzymatic process for the fractionation of baker’s yeast cell wall(Saccharomyces cerevisiae)[J].Food Chemistry,2014,163:108-113.
    [10]
    张莉弘,吴琼,高长城,等.废啤酒酵母细胞超声波破壁工艺优化[J].酿酒科技,2014(2):65-67.
    [11]
    戴宁,张欲中,周东.超高压均质参数对酵母破壁率的影响[J].食品与发酵工业,2011,37(9):109-109.
    [12]
    杜娜,杨学山,韩舜愈,等.葡萄酒泥酵母超氧化物歧化酶分离提取工艺条件优化[J].食品工业科技,2013,34(15):242-245.
    [13]
    徐栋,王春维.高压均质与酶法破碎酵母细胞壁的工艺条件研究[J].饲料工业,2009,30(12):44-47.
    [14]
    范华宁,黄冬云,张晖,等.反复冻融和超声协同作用破碎酵母细胞[J].食品与发酵工业,2013,39(12):62-62.
    [15]
    王慧,程富胜,罗永江,等.响应面法优化酵母多糖的提取工艺[J].食品科学,2012,33(24):93-95.
    [16]
    杨建梅.啤酒废酵母中β-1,3-D-葡聚糖的制备及性质研究[D].泰安:山东农业大学,2012.
    [17]
    张海波,张蔚,张彦,等.QB/T 4572-2013酵母β-葡聚糖[S].中华人民共和国工业和信息化部,2013.
    [18]
    于雅娟,戴军,朱松,等.酸酶水解—HPLC法检测香菇多糖中β-D-葡聚糖含量[J].食品与发酵工业,2012,38(7):148-151.
    [19]
    朱益波,翟丽君,朱明,等.啤酒废酵母中β-D-葡聚糖非降解提取工艺[J].食品科学,2011,32(20):121-125.
    [20]
    GB 5009.5-2003食品中蛋白质的测定[S].
    [21]
    GB/T 5009.6-2003食品中脂肪的测定[S].
    [22]
    Silke C J,Sascha R,Lother W K,et al.Antioxidative activity of(1→3),(1→6)-β-D-glucan from Saccharomyces cerevisiae grown on different media[J].Food Science and Technology,2008,41(5):868-877.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (59) PDF downloads(343) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return