红托竹荪多糖抗衰老和降血糖作用研究
Effects of Dictyophora rubrovalvata polysaccharide on anti- aging and hypoglycemic in mice
-
摘要: 目的:考察红托竹荪多糖（Dictyophora rubrovalvata polysaccharide,DRP）的抗衰老和降血糖作用。方法:采用水提醇沉法提取DRP。采用皮下注射D-半乳糖的方法构建衰老模型小鼠,同时DRP按低剂量（100 mg/kg·d）和高剂量（300 mg/kg·d）灌胃,35 d后测定各组小鼠血清和肝脏中超氧化物歧化酶（SOD）、谷胱甘肽-过氧化物酶（GSH-PX）活性和丙二醛（MDA）含量;给正常小鼠测血糖后皮下注射四氧嘧啶造高血糖模型小鼠,DRP按低剂量（100 mg/kg·d）和高剂量（300 mg/kg·d）灌胃,连续4周,每周测一次空腹血糖值;两组实验均设空白对照组和模型组。结果:给药300 mg/kg·d DRP可以显著增加D-半乳糖所致衰老小鼠血清和肝脏中SOD和GSH-PX活性,显著降低小鼠血清和肝脏中MDA水平;DRP能降低高血糖模型小鼠的空腹血糖,但效果不明显。结论:DRP具有延缓衰老的作用,但降血糖作用不明显。Abstract: Objective: To study anti- aging and hypoglycemic effects of Dictyophora rubrovalvata polysaccharide( DRP) in mice. Methods: DRP was extracted by using the method of water extraction and subsequent alcohol precipitation.The aging model was induced by subcutaneous injection of D- galactose,meanwhile,the mice were given different dose of DRP( 100 mg / kg·d,300 mg / kg·d) by oral gavage.After 35 days,activity of SOD,GSH- PX and concentration of MDA in the serum and liver were determined. Hyperglycemic mice models were established by subcutaneous injection of alloxan.The mice were given different dose of DRP( 100 mg / kg·d,300 mg / kg·d) by oral gavage.Fasting blood glucose was determined once a week. The two tests included blank control group and model group.Results: The activity of SOD and GSH- PX in the serum and liver increased significantly,MDA content decreased significantly.DRP could reduce the fasting blood glucose level of hyperglycemic mice,but the effect was limited.Conclusion: DRP had anti- aging effects on aging model mice but no hypoglycemic effect in mice.
下载: