Fat-soluble alkaloids extracted from Dendrobium Nobile Lindl induced apoptosis of human colorectal cancer HT-29 cells

HE Lei; LUO Jing; WANG Ya-yun; SHI Yu-jie; REN Jian-wu

doi:10.13386/j.issn1002-0306.2017.03.024

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Article Navigation > Science and Technology of Food Industry > 2017 > (03): 170-174

HE Lei, LUO Jing, WANG Ya-yun, SHI Yu-jie, REN Jian-wu. Fat-soluble alkaloids extracted from Dendrobium Nobile Lindl induced apoptosis of human colorectal cancer HT-29 cells[J]. Science and Technology of Food Industry, 2017, (03): 170-174. doi: 10.13386/j.issn1002-0306.2017.03.024

Citation:

HE Lei, LUO Jing, WANG Ya-yun, SHI Yu-jie, REN Jian-wu. Fat-soluble alkaloids extracted from Dendrobium Nobile Lindl induced apoptosis of human colorectal cancer HT-29 cells[J]. Science and Technology of Food Industry, 2017, (03): 170-174. doi: 10.13386/j.issn1002-0306.2017.03.024

Citation:

HE Lei, LUO Jing, WANG Ya-yun, SHI Yu-jie, REN Jian-wu. Fat-soluble alkaloids extracted from Dendrobium Nobile Lindl induced apoptosis of human colorectal cancer HT-29 cells[J]. Science and Technology of Food Industry, 2017, (03): 170-174. doi: 10.13386/j.issn1002-0306.2017.03.024

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Fat-soluble alkaloids extracted from Dendrobium Nobile Lindl induced apoptosis of human colorectal cancer HT-29 cells

doi: 10.13386/j.issn1002-0306.2017.03.024

College of Biological Sciences and Biotechnology,Beijing Forestry University
School of Pharmaceutical science,Peking University Health Science Center

Received Date: 2016-09-12

Abstract

Abstract

Objective: Anti-growth effects of fat-soluble alkaloids extracted from Dendrobium Nobile Lindl on human colorectal cancer HT-29 cells and its possible apoptotic mechanism were studied.Methods: The MTT assay was used to assess the effect of fat-soluble alkaloids extracted from Dendrobium Nobile Lindl on survival rate of HT-29 cells; the morphological changes of HT-29 cells were observed with Hoechst 33342 staining; the levels of intracellular reactive oxygen species( ROS),mitochondrial membrane potential( ΔΨm),cell cycle distribution and apoptosis rate were studied using flow cytometry; western blotting analysis was used to detect the expressions of Caspase-9,3,and intracellular cytochrome C. Results: Fat-soluble alkaloids extracted from Dendrobium Nobile Lindl decreased survival rate of human colorectal cancer HT-29 cells in a doseand time-dependent manner; IC_50 value for 48 h was 0.72 mg / mL. Fat-soluble alkaloids extracted from Dendrobium Nobile Lindl induced cell apoptosis and cell cycle was arrested in G2 phase; it caused decline of ΔΨm and induced ROS accumulation with increased expression levels of apoptotic proteins such as activated Caspase-9,activated Caspase-3,and level of intracellular cytochrome C.Conclusion: Fat-soluble alkaloids extracted from Dendrobium Nobile Lindl can inhibit the growth of HT-29 cells and induce cell apoptosis,which may be associated with the involvement of the mitochondria-mediated apoptotic pathway.
- Dendrobium Nobile Lindl,
- fat-soluble alkaloids,
- HT-29 cells,
- apoptosis,
- reactive oxygen species

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References(29)

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