摘要:
以人参山羊奶粉为研究对象,采用高分辨液质联用技术检测人参山羊奶粉中标志性成分人参皂苷含量,对干、湿法制备的人参山羊奶粉中6种主要的皂苷类活性成分-人参皂苷Rg1、Re、Rb1、Rb2、Rc和Rd的含量进行比较;同时采用皮下注射氢化可的松(50 mg/kg)的方法造肾虚小鼠模型,对小鼠负重游泳时间、累计爬杆时间、血尿素氮(blood urea nitrogen,BUN)、丙二醛(malondialdehyde,MDA)和血乳酸(lactic acid,LA)含量及超氧化物歧化酶(superoxide dismutase,SOD)活力6个指标进行检测;以确定制备方法并评价相应剂型的缓解体力疲劳的功效。结果表明,干法制备的人参山羊奶粉中标志性成分Rg1、Re、Rb1、Rb2、Rc和Rd的含量较高于湿法制备奶粉,为0.09±0.03、0.24±0.05、0.16±0.04、0.08±0.01、0.10±0.02和0.14±0.06 mg/g。体内研究结果表明干法制备人参山羊奶粉低、中、高剂量均具有一定的缓解体力疲劳的作用,在灌胃给予人参山羊奶粉后,低(1.580 g/kg)、中(3.167 g/kg)、高剂量(6.333 g/kg)三个剂量组小鼠负重游泳及爬杆时间均显著优于模型对照组(P<0.05或P<0.01);给予中、高剂量的人参山羊奶粉可显著降低小鼠血清的丙二醛含量及小鼠游泳10 min后全血乳酸含量(P<0.05),其中高剂量人参山羊奶粉能显著降低小鼠游泳休息20 min后全血乳酸含量(P<0.05);中、高剂量组均能显著降低小鼠的血清尿素氮含量,提高小鼠的超氧化物歧化酶活力(P<0.05或P<0.01)。干法制备的人参山羊奶粉具有增强机体对运动负荷的适应能力,可后续开发成抗疲劳的保健食品。
Abstract:
Taking ginseng goat milk powder as the research object,rapid-resolution liquid-mass technology was used to determine the marker components in ginseng goat milk powder. The contents of ginsenoside Rg1,Re,Rb1,Rb2,Rc and Rd were compared among the six main saponins active components of ginseng goat powder prepared by dry and wet method. At the same time,the mouse model of kidney deficiency was made by subcutaneous injection of hydrocortisone(50 mg/kg)and the six indexes of weight-bearing swimming time,cumulative climbing time,blood urea nitrogen(BUN),malondialdehyde(MDA),lactic acid(LA)content and superoxide dismutase(SOD)activity were measured. To determine the preparation method and to investigate the efficacy of the corresponding dosage form of anti-fatigue. The results showed that the contents of Rg1,Re,Rb1,Rb2,Rc and Rd in ginseng goat milk powder prepared by dry method were 0.09±0.03,0.24±0.05,0.16±0.04,0.08±0.01,0.10±0.02 and 0.14±0.06 mg/g,respectively,which were higher than those in milk powder prepared by wet method. The in vivo results showed that low,medium and high dose had a certain effect on anti-fatigue. The weight-bearing swimming and climbing time of mice in three groups-low(1.580 g/kg),medium(3.167 g/kg)and high dose(6.333 g/kg)were significantly better than those in model control group(P<0.05 or P<0.01). Medium and high dose could significantly decrease the content of malondialdehyde in serum and lactic acid content after swimming for 10 min(P<0.05)and high dose could significantly decrease the content of lactic acid after swimming rest 20 min(P<0.05).Medium and high dose could significantly decrease the content of serum urea nitrogen and increase the activity of superoxide dismutase in mice(P<0.05 or P<0.01). Ginseng goat milk powder prepared by the dry method can significantly enhance the adaptation ability of the body to exercise load and can be developed into anti-fatigue health food.
百度学术
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