摘要:
采用超声波-微波协同提取的方法,通过单因素和正交试验对提取时间、微波功率和超声波功率进行研究,优化香菇粉中麦角甾醇和维生素D2(VD2)的提取工艺。结果表明,各因素对麦角甾醇和VD2提取效率的影响程度依次为提取时间>微波功率>超声波功率,最终优化提取条件为:无水乙醇为提取溶剂,提取时间20 min,料液比1:20(g/mL),提取温度为室温,超声波功率为400 W,微波功率为200 W。在该提取条件下,麦角甾醇含量高达(3.59±0.69)mg/g,VD2含量高达(123.52±0.69)μg/g。麦角甾醇的平均回收率(n=6)为114.57%,RSD(n=6)为3.83%,VD2平均回收率(n=6)为102.26%,RSD(n=6)为4.95%。该法同比已有方法提取效率高,提取和分析时间短,操作简便,且具有较好的准确性及重现性。
Abstract:
The main factors affecting the ultrasonic-microwave synergistic extraction efficiency of extraction time,microwave power,and ultrasonic power on the yield of ergosterol and vitamin D2(VD2)in shiitake mushroom powder were investigated by single factor and orthogonal experiments. The results showed that the factors affecting the extraction efficiency of ergosterol and VD2 were as follows:Extraction time>microwave power>ultrasonic power. The optimal extraction conditions were absolute ethanol as extraction solvent,extraction time was 20 min,ratio of material to liquid was 1:20 (g/mL),the extraction temperature was room temperature,the ultrasonic power was 400 W,and the microwave power was 200 W. Under the extraction conditions,the ergosterol content was as high as(3.59±0.69) mg/g,and the VD2 content was as high as(123.52±0.69) μg/g. The average recovery of ergosterol(n=6)was 114.57%,RSD(n=6)was 3.83%,VD2 average recovery(n=6)was 102.26%,RSD(n=6)was 4.95%. The method showed higher extraction efficiency,shorter extraction and analysis time,simpler operation,and better accuracy and reproducibility compared with reported methods.
百度学术
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