摘要:
为进一步探究无柄灵芝提取物中营养成分及其抗氧化能力。本研究以赤芝为对照,先用蒸馏水和55%的乙醇按照一定比例提取获得相应的水提物和醇提物,接着通过苯酚-硫酸法、香草醛-冰醋酸法分别测定4种提取物中的总糖和总三萜含量。再利用L-8900型氨基酸自动分析测定游离氨基酸含量。最后检测4种提取物分别对2,2'-联氨-二-3-基-苯并噻唑-6-磺酸(2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS)、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)、羟基自由基的清除能力。结果表明,无柄灵芝与赤芝水提物总糖含量均显著高于相应的醇提物(P<0.05),无柄灵芝醇提物总糖含量为48.77%,赤芝醇提物总糖含量为51.54%,两种灵芝水提取物之间的总糖含量没有显著性差异(P>0.05);无柄灵芝醇提物总三萜含量显著高于其它3种提取物(P<0.05),达25.37 mg/g,但其水提物三萜含量却是最低,仅1.30 mg/g。无柄灵芝水提物中游离必需氨基酸总含量最高,为45.542 mg/g,而赤芝水提物含量最低,为2.381 mg/g。无柄灵芝醇提物对ABTS、DPPH、羟基自由基清除率均最高,分别为54.48%、24.42%、10.32%,说明其抗氧化能力较强。本研究可为无柄灵芝的进一步开发应用提供理论依据。
关键词:
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无柄灵芝
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赤芝
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三萜
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游离氨基酸
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抗氧化
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总糖
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醇提物
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水提物
Abstract:
In order to further explore the nutritional components and antioxidant capacity of G. resinaceum extract,G. lucidum was used as a control in this study. First,distilled water and 55% ethanol were used to extract the corresponding water extracts and alcohol extracts,and then the total sugar and total triterpene content in the four extracts were determined by the phenol-sulfuric acid method and the vanillin-glacial acetic acid method,respectively. Next the content of free amino acids was determined by the L-8900 automatic amino acid analyzer. Finally,the 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS),1,1-diphenyl-2-picrylhydrazyl(DPPH),and hydroxyl radical scavenging capacity of 4 kinds of extracts were measured. The results showed that the total sugar content in the water extract of G. lucidum and G. resinaceum were significantly higher than the corresponding alcohol extract(P<0.05). The total sugar content of G. resinaceum alcohol extract was 48.77%,and the total sugar content of G. lucidum alcohol extract was 51.54%,respectively. There was no significant difference in the total sugar content between the two water extracts(P>0.05). The content of total triterpenes in alcohol extracts of G. resinaceum was significantly higher than that of the other three extracts(P<0.05),reaching 25.37 mg/g,but the triterpene content of the water extract was the lowest,only 1.30 mg/g. The highest content of free essential amino acid in G. resinaceum water extract was 45.542 mg/g,while the content of G. lucidum water extract was the lowest(2.381 mg/g). G. resinaceum had the highest scavenging rates of ABTS,DPPH and hydroxyl radicals,which were 54.48%,24.42% and 10.32%,respectively. It indicated that G. resinaceum had strong antioxidant capacity. This study can provide a theoretical basis for the further development and application of G. resinaceum.
百度学术
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