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中国精品科技期刊2020
张文开,鲍征宇,Diego Armando PINZON NUNEZ,等. 超声辅助乙醇提取富硒金花葵花朵中的硒及抗氧化活性分析[J]. 食品工业科技,2024,45(16):201−208. doi: 10.13386/j.issn1002-0306.2023070202.
引用本文: 张文开,鲍征宇,Diego Armando PINZON NUNEZ,等. 超声辅助乙醇提取富硒金花葵花朵中的硒及抗氧化活性分析[J]. 食品工业科技,2024,45(16):201−208. doi: 10.13386/j.issn1002-0306.2023070202.
ZHANG Wenkai, BAO Zhengyu, Diego Armando PINZON NUNEZ, et al. Ultrasonic Assisted Ethanol Extraction of Selenium and Antioxidants from Selenium-rich Hibiscus manihot L.[J]. Science and Technology of Food Industry, 2024, 45(16): 201−208. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070202.
Citation: ZHANG Wenkai, BAO Zhengyu, Diego Armando PINZON NUNEZ, et al. Ultrasonic Assisted Ethanol Extraction of Selenium and Antioxidants from Selenium-rich Hibiscus manihot L.[J]. Science and Technology of Food Industry, 2024, 45(16): 201−208. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070202.

超声辅助乙醇提取富硒金花葵花朵中的硒及抗氧化活性分析

Ultrasonic Assisted Ethanol Extraction of Selenium and Antioxidants from Selenium-rich Hibiscus manihot L.

  • 摘要: 为促进优质天然富硒植物资源的科学开发利用,本文选取富硒金花葵为研究对象,重点研究了其不同器官中硒的分布、含有机硒活性成分提取条件和抗氧化活性,并探讨了植物源有机硒活性成分的药用价值和经济价值。采用氢化物发生-原子荧光光谱法(Hydride Generation-Atomic Fluorescence Spectrometry,HG-AFS)检测样品中的硒含量,采用超声波辅助乙醇提取金花葵花朵中的含硒有机活性组分并使用响应面法优化提取工艺,同时对含硒提取物开展抗氧化活性研究。结果显示,金花葵各器官中硒含量从高到低依次为叶、果、花、根和茎,分别为21.99、14.29、12.93、10.90和10.23 mg/kg。响应面优化结果表明,含硒有机活性成分最佳提取参数为提取温度40 ℃、固液比1:60、提取时间40 min、超声功率350 W、乙醇浓度50%(V/V)和提取次数1次。在最佳提取参数下,硒的提取率为35.65%±0.65%。DPPH抗氧化活性测定结果显示,含硒有机活性成分的半抑制浓度(IC50)为0.048 mg/mL(硒元素浓度为0.79 μg/mL),羟基自由基清除实验显示样品浓度为8 mg/mL时达到最大清除率97%,IC50为0.56 mg/mL,还原力实验表明样品的还原能力随样品浓度的增大而增强。证明金花葵的含硒乙醇提取物具有良好的抗氧化能力。本研究可以为合理利用富硒金花葵和研发加工富硒农副作物提供一些理论依据。

     

    Abstract: To promote scientific progress and optimize the utilization of natural plant resources that are rich in selenium. Specifically, it explored the distribution of selenium in the plant organs of Hibiscus manihot L., the variables that impact its selenium-containing organic components, the antioxidant potential of these components, and the health and economic advantages of its selenium-rich extracts. The analysis of the total Se concentration in the samples was conducted using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS). The organic components containing Se in Hibiscus manihot L. flowers were extracted using the ultrasound-assisted extraction (UAE) method with ethanol, and the antioxidant activity of the extracts was examined. The factors were optimized using response surface methodology (RSM). The results showed that the total Se concentrations in different organs of Hibiscus manihot L., from highest to lowest, were found in the leaves, fruits, flowers, roots, and stems, with concentrations of 21.99, 14.29, 12.93, 10.90, and 10.23 mg/kg, respectively. The RSM optimization results indicated that the ideal conditions for the extraction parameters were as follows: extraction temperature of 40 ℃, solid-liquid ratio of 1:60, extraction time of 40 minutes, ultrasonic power of 350 W, ethanol concentration of 50% (V/V), under one-time extraction. For the optimal conditions, the selenium extraction rate was 36.65%±0.65%. The DPPH antioxidant activity analysis showed a half-inhibitory concentration (IC50) value of 0.048 mg/mL (with a Se concentration of 0.79 μg/mL), the hydroxyl radical scavenging analysis showed that the maximum clearance rate was 97% when the extracts concentration was 8 mg/mL, and the IC50 was 0.56 mg/mL. The reducing power analysis showed that the reducing power of the extracts increases with the increase of the concentration, indicating that the extracts possess a good antioxidant capacity. The present study provides empirical evidence to support the efficient use of selenium-rich Hibiscus manihot L. and the extraction of selenium-rich agricultural by-products.

     

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