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中国精品科技期刊2020
李雨欣,胡芸利,刘聪,等. 河套麦胚多肽的制备工艺优化及其体外降血脂活性[J]. 食品工业科技,2024,45(17):1−7. doi: 10.13386/j.issn1002-0306.2023090197.
引用本文: 李雨欣,胡芸利,刘聪,等. 河套麦胚多肽的制备工艺优化及其体外降血脂活性[J]. 食品工业科技,2024,45(17):1−7. doi: 10.13386/j.issn1002-0306.2023090197.
LI Yuxin, HU Yunli, LIU Cong, et al. Preparation Optimization of Hetao Wheat Germ Polypeptide and Its in Vitro Hypolipidemic Activity[J]. Science and Technology of Food Industry, 2024, 45(17): 1−7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090197.
Citation: LI Yuxin, HU Yunli, LIU Cong, et al. Preparation Optimization of Hetao Wheat Germ Polypeptide and Its in Vitro Hypolipidemic Activity[J]. Science and Technology of Food Industry, 2024, 45(17): 1−7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090197.

河套麦胚多肽的制备工艺优化及其体外降血脂活性

Preparation Optimization of Hetao Wheat Germ Polypeptide and Its in Vitro Hypolipidemic Activity

  • 摘要: 本文以河套麦胚蛋白为原料,采用碱性蛋白酶对其进行酶解,以水解度为指标,通过单因素及正交试验优化其工艺条件,并进行体外降血脂作用研究。实验结果表明,在底物浓度为6.0%,温度为50 ℃、pH为10.5及加酶量为12000 U/g的条件下,麦胚蛋白的水解度达到37.18%±0.25%;麦胚多肽5 mg/mL时胆固醇胶束溶解度抑制率为27.11%±0.36%,胰脂肪酶活性抑制率为33.54%±0.57%,胆固醇酯酶活性抑制率为38.13%±0.29%;超滤后相对分子质量小于1 kDa的多肽组分占54.30%±0.32%,说明小分子量的胚芽多肽可能具有较好的降血脂作用。上述结果为进一步研究河套胚芽多肽降血脂提供了理论依据。

     

    Abstract: In this paper, alkali protease was used to enzymatically hydrolyze wheat germ proteins grown in the Hetao region. A univariate analysis and an orthogonal experiment were conducted to optimize the enzymatically hydrolyze process conditions of wheat germ proteins, with the degree of hydrolysis taken as the indicator. Moreover, the in vitro hypolipidemic effect of wheat germ polypeptides was investigated. The experimental results showed that the degree of hydrolysis of wheat germ proteins was 37.18%±0.25% achieved by the following conditions, substrate concentration of 6.0%, temperature at 50 ℃, pH of 10.5 and protease amount of 12000 U/g. When the concentration of wheat germ polypeptides was 5 mg/mL, the rate of inhibiting cholesterol solubilisation into micelles was 27.11%±0.36%, the pancreatic lipase activity inhibition rate was 33.54%±0.57%, and cholesterol esterase activity inhibition rate was 38.13%±0.29%. Moreover, after ultrafiltration, the relative molecule weight of polypeptides less than 1 kDa accounted for 54.30%±0.32% of all polypeptides, indicating that low-molecular-weight wheat germ polypeptides might yield superior hypolipidemic effects. The work provides a theoretical foundation for further exploring the mechanism between Hetao wheat germ polypeptides and hypolipidemic effect.

     

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